Figure 4.

Evidence of hepatic mitochondrial dysfunction in mice sensitized by PNT. Hepatic mitochondrial state 3 and 4 respiration rates in the presence of succinate (A) or palmitoyl-carnitine (B) were evaluated, and the relative respiratory control ratios (RCRs) were shown (upper panels). Oxygen consumption in the presence of oligomycin or uncoupled by FCCP (C), and degree of coupling values (D) were also shown. The mitochondrial H₂O₂ yield (E), the SOD activity (F), the basal and total activity of the aconitase (G) and its ratio (H) were reported. Data are reported as means ± SEM from duplicate analyses. Data were analyzed with independent sample t-test. CTRL = control mice; PNT = mice sensitized with peanut extract. * vs. CTRL, p < 0.05; ** vs. CTRL, p < 0.0001.