Table 2.
Primer list.
| Gene | NCBI Reference Sequence | Forward Primer | Reverse Primer |
|---|---|---|---|
| Tight Junction | |||
| CLDN1 | NM_016674.4 | TGTGGATGGCTGTCATTGGGG | ATTCATACCTGGCATTGATGGGGG |
| CLDN2 | NM_016675.4 | ACGGCTCCGTTTTCTAGATGCC | CGTTTGGCTGCTGCTCTTGC |
| CLDN3 | NM_009902.4 | AGCCCTCATCGTGGTGTCCA | GGCCGTCTCGTCTTGTACGC |
| CLDN4 | NM_009903.2 | GAGCCGTGTTCATCGTGGCA | CCCAGCCGACGTAAAGCGAG |
| CLDN5 | NM_013805.4 | GTGCGTGGTGCAGAGTACCG | GAGCGCCGGTCAAGGTAACA |
| CLDN8 | NM_018778.3 | ATGCACGGGGGACGATGAGA | TGAGCACAACCAAGCCGGTG |
| OCLN | NM_008756.2 | ACGGTCCTCCTGGCTCAGTT | GATAAGCGAACCTTGGCGGC |
| TJP1 | NM_001163574.1 | TGTTTATGCGGACGGTGGCG | TCCATTGCTGTGCTCTTAGCGG |
| Inflammation | |||
| CCL2 | NM_011333.3 | TTAAAAACCTGGATCGGAACCAA | GCATTAGCTTCAGATTTACGGGT |
| CCR2 | NM_009915.2 | ATAAAGGAGCCATACCTGTAAATGC | CATGTGGTGAATCCAATGCCCT |
| IL1B | NM_008361.4 | TGCCACCTTTTGACAGTGATGAGA | TGTTGATGTGCTGCTGCGAGA |
| IL6 | NM_031168.2 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC |
| TLR2 | NM_011905.3 | AGGAGGTGCGGACTGTTTCCT | ATTTGACGCTTTGTCTGAGGTTTCG |
| TLR4 | NM_021297.3 | TCCCTGCATAGAGGTAGTTCCTA | TTCAAGGGGTTGAAGCTCAGA |
| TNF | NM_001278601.1 | TCGGTCCCCAAAGGGATGAGA | GGTGGTTTGTGAGTGTGAGGGT |
| VCAM1 | NM_011693.3 | ACGTGGACATCTACTCTTTCCCCA | CTTGACCGTGACCGGCTTCC |
| NFKB1 | NM_008689.2 | CTGCCATGTCTGCTGCTGCT | CGTGGGCATCACCCTCCAGA |
| Housekeeping | |||
| HPRT | NM_013556.2 | TCCCAGCGTCGTGATTAGCG | TCGAGCAAGTCTTTCAGTCCTGT |