Fig. 5. Confocal images of 293T cells. (A) The cells were treated with Ac4GalNAz followed by a click reaction with alkyne-functionalized DNA and RCA (PMT 700 V, gain 2, and offset –30); (B) the cells were transfected with HA-tagged GPC3 and stained by an anti-HA antibody and a TRITC-conjugated secondary antibody (PMT 700 V, gain 2, and offset –30); (C) both processes (A) and (B) were performed (PMT 700 V, gain 2, and offset –30); (D) similar to process (C) but without RCA (PMT 850 V, gain 2, and offset –30). Scale bars: 20 μm.