Table 2.

Comparison of translation inhibition assays.

Method Name		Advantages	Disadvantages	References
In vitro assays	Radioactive amino acid incorporation	First assay available for RIP activity.	Requires handling of radioactive materials.	[66,67,68,69]
	Luciferase synthesis	Adaptable to HTS. No radioactive materials.	Luciferase can be subject to interference by small inhibitor molecules leading to false positives.	[71,73,80]
Mammalian cell-based assays	In-cell radioactive amino acid incorporation	Adaptable to HTS.	Requires handling of radioactive materials.	[43]
	In-cell luciferase synthesis	Adaptable to HTS. No radioactive materials.	Requires transfection of cells. Low sensitivity, high background and high sample-to-sample variation.	[44,74]
	Cell-based luciferase assay	Adaptable to HTS. No radioactive materials. No cell transfection.	Can yield nonspecific inhibitors.	[72]
	In-cell GFP synthesis	Highly sensitive. Easy detection of GFP.	Requires cell transfection.	[39,40,75]

GFP: green fluorescent protein.