Fig. 1. Schematic of the metabolic labelling of H. pylori CGds. 17β-([3′′-Azidopropoxy)-5-androsten-3β-ol (1; an azide-containing analogue of cholesterol) was added to H. pylori culture and incorporated into the biosynthetic pathway to produce various CGd analogues. After harvest and Folch extraction, these analogues were further reacted with a fluorescent alkyne (
) via Cu(i)-catalysed 1,3-dipolar cycloaddition to obtain the conjugated products, which were then analysed by HPLC and MS.
