Figure 2.

Phage HK620 in vivo infection assays. (A) In vivo plaque forming assay after incubation of 4.5 × 10³ pfu mL⁻¹ HK620 particles with 25 µg mL⁻¹ LPS from E. coli H TD2158 LPS (green). After 0, 10, 30, 120, 240, 300 min (individual bars in each experiment) the reaction mix (450 pfu) was plated on E. coli H TD2158 and plaques were determined after overnight incubation at 37 °C. Controls: Phage buffer (grey), 25 µg mL⁻¹ polysaccharide (dark blue), lipid A (cyan) or E. coli H TD2158 LPS pretreated with HK620TSP (red). Standard deviations are from three independent experiments. (B) Time course of OD₆₀₀ of cultures at 37 °C of E. coli strains H TD2158 (green), H TD2158 ΔOmpA (purple) or H TD2158ΔOmpC (red) after addition of phage HK620 at OD 0.3 with a multiplicity of infection of 0.1. No phage was added to a control with E. coli H TD2158 (black).