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. 2018 Jun 18;10(6):334. doi: 10.3390/v10060334

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Recombinant Pr77^Gag-His₆-tag fusion protein expressed in soluble fraction of E. coli. (A) SDS-PAGE analysis showing recombinant MMTV Pr77^Gag-His₆-tag fusion protein expression in the bacterial soluble fraction (lane 4) transformed with AK1; (B) western blot analysis of MMTV Pr77^Gag expression by AK1 in soluble fraction analyzed with an α-His₆ monoclonal antibody (lane 4); and (C) with an α-p27 monoclonal antibody (lane 4), respectively.