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. 2018 Jun 18;10(6):334. doi: 10.3390/v10060334

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Silent mutations in the Shine-Dalgarno-like sequence and the predicted relative translation rates from the first and the second in-frame ATGs. (A) Illustration of the 18-nucleotide region mutated in MMTV gag gene to disrupt the Shine-Dalgarno-like sequence (underlined) 4 nts upstream of the second in-frame ATG (at nucleotide position 1674). These mutated sequences were cloned in both with and without His-tag clones AK7 and AK31, respectively; (B) Bar graphs showing the predicted translation rates from the legitimate first start codon and the second in-frame start codon in the wild type and in AK7(His+) and AK31(His−)-containing a mutated Shine-Dalgarno-like sequence.