Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jun 18;10(6):334. doi: 10.3390/v10060334

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Expression of recombinant Pr77^Gag-His₆-tag fusion protein from AK7 in soluble fractions of E. coli before and after immobilized metal affinity chromatography (IMAC) purification. (A) SDS-PAGE analysis showing recombinant MMTV Pr77^Gag-His₆-tag fusion protein expressed in the bacterial soluble fractions transformed with AK7 (lane 3), followed by IMAC purification (lane 4); (B) western blot analysis of MMTV Pr77^Gag-His₆-tag fusion protein analyzed with an α-His₆ monoclonal antibody; and (C) with α-p27 monoclonal antibody, respectively.