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. 2018 Jun 18;10(6):334. doi: 10.3390/v10060334

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Resolution of IMAC-purified recombinant Pr77^Gag-His₆-tag fusion protein by size exclusion chromatography and western blot analysis. (A) Absorbance versus elution time chromatogram of eluted fractions after size exclusion chromatography; (B) Coomassie Brilliant Blue-stained SDS-PAGE analysis of peak fractions 23 to 27, showing the resolution of purified recombinant MMTV Pr77^Gag expressed from AK7; (C) western blot analysis of pooled peak fractions of purified MMTV Pr77^Gag-His₆-tag fusion protein analyzed with α-His₆ and α-p27 monoclonal antibodies, respectively.