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Transmission electron micrographs showing virus-like particles (VLPs) following in vitro assembly. (A–D) In vitro assembled VLPs from purified recombinant Pr77Gag-His6-tag fusion expressed from AK7 in the presence of yeast tRNA; and (E,F) negative controls consisting of assembly buffer and yeast tRNA in the absence of any protein (Scale bar = 50 nm, 135,000× magnification).
