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Formation of VLPs by recombinant Pr77Gag-His6-tag fusion in E. coli BL21(DE3). Transmission electron micrographs showing VLPs assembled from (A) recombinant Pr77Gag-His6-tag fusion expressed in E. coli BL21(DE3) cells transformed with AK7 and (B) AK31 (without His6 tag); (C,D) un-induced BL21(DE3) cells transformed with AK7 and AK31, respectively (Scale bar = 100 nm; 60,000× magnification).
