Figure 2.
Seven-day growth characterization of the OAZ1- cell lines, 775–1 and 775–3, and the parental cell line. (a) Proliferation and viability of the OAZ1- cell lines 775–1 (●), 775–3 (▲), and the parental (■) cell line. Cells were plated at a density of 7 × 105 cells in T-25 flasks and enumerated using trypan blue exclusion over a seven-day period; and (b) glucose and lactate concentration in the culture media quantified using a bioanalyzer over the same seven-day period. Data from two independent growth studies are shown, with error bars representing the standard deviation.