Table 2.

Primer sequences and annealing temperature of PCR amplification of specific bacterial.

Bacterial Species	Primer Sequences (5′­3′)	Lengths (bp)	Annealing Temperature (°C)
Escherichia coil	F: 5′-GTTAATACCTTTGCTCATTGA-3′	340	51
	R: 5′-ACCAGGGTATCTTAATCCTGTT-3′
Enterococcus	F: 5′-ACTCGTTGTACTTCCCATTGT-3′	144	52
	R:5′-CCCTTATTGTTAGTTGCCATCATT-3′
Bifidobacterium	F: 5′-GGGTGGTAATGCCGGATG-3′	442	61
	R: 5′-TAAGCGATGGACTTTCACACC-3′
Lactobacillus	F: 5′- AGCAGTAGGGAATCTTCCA-3′	341	55
	R: 5′-CACCGCTACACATGGAG-3′
Bacteroides fragilis	F: 5′- CTGAACCAGCCAAGTAGCG-3′	230	62
	R:5′-CCGCAAACTTTCACAACTGACTTA-3′
Clostridium tender	F: 5′-GCACAAGCAGTGGAGT-3′	246	58
	R: 5′-CTTCCTCCGTTTTGTCAA-3′