Table 2.

Testing the reactivation and the reusability of BBP as a mycotoxin binder: Extraction of ZEN by BBP and elution of the mycotoxin from BBP by ethanol-water mixture.

Number of Applications	Procedure Performed	ZEN (%) in the Buffer (A) or the Eluent (B, C)	∑ (%)
1st application of the polymer	A: After extraction with BBP	8.0 ± 0.3	99.8
	B: After the 1st elution	85.8 ± 1.6
	C: After the 2nd elution	6.0 ± 0.7
2nd application of the polymer	A: After extraction with BBP	7.1 ± 0.2	98.3
	B: After the 1st elution	84.8 ± 1.5
	C: After the 2nd elution	6.4 ± 0.8
3rd application of the polymer	A: After extraction with BBP	7.4 ± 0.4	-

A: Percent of ZEN (10 μM) remaining in 1.5 mL sodium acetate buffer (pH 5.0), after its incubation with 10 mg BBP for 40 min at 25 °C. B: Percent of ZEN recovered in the ethanol-water mixture, after the first elution from the polymer with 1.5 mL 50 v/v% ethanol for 10 min at 25 °C. C: Percent of ZEN recovered in the ethanol-water mixture, after the second elution from the polymer with 1.5 mL 50 v/v% ethanol for 10 min at 25 °C.