. 2001 Oct 1;29(19):4035–4042. doi: 10.1093/nar/29.19.4035

Table 1. Overexpression of FTF or FTFΔLBD increases 12α-hydroxylase promoter–luciferase construct activity in Hep3B cells.

Expression plasmid	Promoter activity		Regulation (%)
	–CDCA	+CDCA
Control (GHR)	100	102 ± 15	100 (3)
Wild-type FTF	769 ± 362	432 ± 161	58 ± 8 (4)^a
HNF-4	199 ± 34	276 ± 96	131 ± 32 (3)^b
FTFΔLBD	296 ± 137	316 ± 155	106 ± 10 (3)^b
FTFΔAF-2	ND	ND	ND

pCMV-GHR, as a control, pCI-FTF, pCI-HNF-4, pCI-FTFΔLBD or pCI-FTFΔAF-2 was co-transfected into Hep3B cells together with pGL3-R12α-865. Cells were treated with or without 100 µM CDCA, harvested and analyzed for luciferase and β-galactosidase activities as described in Materials and Methods. The data were normalized to the activity produced by construct pGL3-R12α-865 co-transfected with the control plasmid pCMV-GHR in the absence of CDCA and represent the averages of (n) experiments ± SD. ND, not detectable.

^aDiffers from the control (P < 0.05).

^bDoes not differ from the control (P > 0.1).