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. 2018 Jun 1;10(6):225. doi: 10.3390/toxins10060225

Figure 4.

Figure 4

Detection and neutralization of CDTaN fusion toxins. (A) Binding of CDTa and CDTaN–TcdB1–543 to the outer cell surface detected by immunoblot analysis using anti-CDTa rabbit serum; (B) Binding of all CDTaN fusion toxins and of TcdB1–543. Shown is the detection by TcdB1–540-specific scFv-Fc VIF090_A6 (left panel) and by anti-CDTa rabbit serum (right panel); (C) Inhibition of pH-dependent delivery of CDTa and CDTaN–TcdB1–543 by the v-ATPase inhibitor bafilomycin A1; (D) Neutralization of CDTaN–TcdB1–543 by anti-CDTa rabbit serum within the cell culture assay. Scale bars in controls C and D represent 20 µm.