Table 1.
Primers used for cloning of the CDTa and CDTb constructs.
| Name | Base Sequence (3’-5’) | |
|---|---|---|
| pGEX-2T constructs | CDTa (aa 1–463) s | TAGGATCCAAAAAATTTAGGAAACATAAAAGGATTAG |
| CDTa (aa 44–463) s | TTAGGATCCGTTTGCAATACTACTTACAAGGC | |
| CDTa (aa 1–463) a | ATGAATTCTTAAGGTATCAATGTTGCATCAAC | |
| CDTb (aa 1–876) s | TAAGATCTAAAATACAAATGAGGAATAAAAAGGTATTAAG | |
| CDTb (aa 43–876) s | TAAGATCTGAAATTGTAAATGAAGATATACTCCC | |
| CDTb (aa 1–876) a | ATGAATTCCTAATCAACACTAAGAACTAATAACTCTC | |
| pQE30 constructs | CDTaN Bam s | AATGGATCCGTTTGCAATACTACTTACAAGG |
| CDTaN Kpn a | AATGGTACCATCATCTTTAAAATCAAGACTATTTAC | |
| GTD TcdB Kpn s | AATGGTACCATGAGTTTAGTTAATAGAAAACAGTTAG | |
| GTD TcdB Hind a | AATAAGCTTTTAAAGAGAACCTTCAAAATAATTCCTTTTATATTC | |
| GTD TcdA Kpn s | AATGGTACCATGTCTTTAATATCTAAAGAAGAG | |
| GTD TcdA Hind a | AATAABCTTTTAAAGAGATCCACCAGTATAATCTC | |
| GTD TcnA Kpn s | AATGGTACCATGCTTATAACAAGAGAACAATTAATG | |
| GTD TcnA Hind a | AATAAGCTTTTAGAGAGTTCTTCCTATATAAGTTTTTATC | |
| GTD TcsH Kpn s | AATGGTACCATGTCTTTAATATCTAAAGATGAATTAATAAAAC | |
| GTD TcsH Hind a | AATAAGCTTTTAAAGAGATTCCTGAGTATAATCTCTTAC | |
| GTD TcsL Kpn s | AATGGTACCATGAACTTAGTTAACAAAGCCC | |
| GTD TcsL Hind a | AATAAGCTTTTAAAGTGCACCTTCAAAATAACC |