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. 2018 Jun 7;10(6):312. doi: 10.3390/v10060312

Figure 3.

Figure 3

MK1-OSU cells infected with influenza A (MN08 and IA07) virus. (A) Cells were infected with MN08 or IA07 viruses at MOI of 0.01 and incubated for 24 h at 37 °C and were fixed with 2% paraformaldehyde in PBS. The fixed cells were incubated with mouse anti-influenza A nucleoprotein and were stained with goat anti-mouse IgG-Alexa 488 (green color) for 1 h. Nuclei were stained using DAPI (blue color) and cells were washed with PBS and examined under an inverted Olympus AX70 fluorescent microscope at 20× magnification. (B) Percentage of MK1-OSU cells infected with influenza A (MN08 and IA07) virus. MK1-OSU were infected at MOI of 0.01 and incubated for 24 h at 37 °C. Following incubation, cells were fixed and permeabilized using BD Cytofix/Cytoperm™. After blocking with 1% goat serum, cells were incubated with primary antibodies against the nucleoprotein of influenza A virus and were stained using goat anti-mouse IgG-Alexa 488. Percentage of cells and mean fluorescence intensity were measured using a FACSCalibur cytometer. Values represent the average for three experiments ± SE.