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. 2018 Jun 7;10(6):312. doi: 10.3390/v10060312

Figure 6.

Figure 6

Gene expressions of TLRs and RLRs in MK1-OSU and SD-PJEC cells 24 h post-infection determined using real-time RT_PCR. MK1-OSU cells were infected with MN08 or IA07 viruses at MOI of 0.01 and incubated for 24 h at 37 °C. Values represent the mean ± SE of ΔCt (Ct of target—Ct of housekeeping gene) for three experiments. The difference in the Ct values has an inverse relationship with the gene expression. (A) TLR-7 mRNA increased at 24 h in SD-PJEC cells infected with IA07. (B) MDA5 gene expression increased in MK1-OSU cells infected with MN08 at 24 h. (C) No difference in the RIG-I mRNA level due to SIV infection in both cell types. p < 0.05 (*) represented a significant difference between uninfected and infected cells.