Figure 2.

Resolution of a synthetic 28mer (5′-CCA TCC ACT ACA ACT ACA TGT GTA ACA G-3′) as a function of laser energy (µJ) for selected sugar additives (4 g/l) added to the 3-HPA matrix (35 g/l). Each additive–matrix combination plus analyte was manually dispensed as five different spots and screened by manual data acquisition. From left to right are standard matrix without additive (S) and matrix with fructose, glucose, sucrose, n-acetylglucosamine, fucose, trehalose and glucosamine as additives, respectively.