Table 1. Comparison of automatic data acquisition mode analysis of single base extension products of an A/T mutation with different 3-HPA matrices.
Matrix |
Valley between heterozygote A/T peaks (%)a |
Failed sample spots (%)b |
Standard 3-HPA | 85.3 ± 5.1 | 23.9 |
Fucose-doped 3-HPA | 67.3 ± 6.7 | 11.5 |
96 MALDI samples were spotted on SpectroCHIPs and analyzed in automatic data acquisition mode.
a100% corresponds to no resolution between the two peaks (see Figs 6 and 7).
bPercentage of spots not resolved where the two peaks were detected as only one broad peak.