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. 2018 Jun 9;8(6):103. doi: 10.3390/brainsci8060103

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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High-power image of vesicular GABA transporter (VGAT)-Venus and CTβ-Alexa 594 labeling in the IC. (A) Venus expression (green) was found in both large and small, disc-shaped and stellate neurons in the IC, which we have previously demonstrated to be primarily GABAergic [31]; (B) CTβ-Alexa 594 (red) labeling appeared as small vesicular shaped puncta within cell bodies; (C) The distinct appearance of the two labels enabled ready identification of retrograde-only (red arrow), Venus-only (green arrow), and double-labeled cells (yellow arrow).