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. 2018 Jun 4;10(6):180. doi: 10.3390/cancers10060180

Figure 3.

Figure 3

Knocking-down KIAA0100 reduces cell aggregation and viability in suspension. MDA-MB-231 cells were reverse transfected with KIAA0100 siRNA and maintained in suspension culture plate coated with poly-HEMA. (A) 24 h and 48 h after transfection, both mRNA and protein levels of KIAA0100 in cell treated with KIAA0100 siRNA were examined by qPCR and Western blot. Both mRNA and protein levels were significantly reduced compared to the cells treated with negative siRNA as well as mock controls (*, p < 0.05); (B) cell aggregation in suspension upon KIAA0100 silencing was significantly reduced compared to the cells treated with negative siRNA and mock control; (C) no significant difference in total cell number counts between cells treated with KIAA0100 siRNA and controls in 24 and 48 h after the treatment by ViaCell (p > 0.05); (D) cell Viability (in percentage) in 24 and 48 h after treatment. No significant cell viability difference in 24 h (p > 0.05). However, silencing the expression of KIAA0100 resulted in significant drop in cell viability 48 h after treatment (*, p < 0.05).