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. 2018 Jun 4;10(6):180. doi: 10.3390/cancers10060180

Figure 5.

Figure 5

Knocking-down KIAA0100 induces anoikis. (A) Annexin V staining: anoikis of the cell in suspension were assessed by Annexin V-FITC apoptosis assay. Most of the mock control and negative siRNA transfected cells show no visible signal of Annexin V-FITC other than sporadic death cell stained with propidium iodide (PI) (white arrow). Two types of staining pattern for Anoikis were shown for cells transfected with KIAA0100 siRNA: early stage of Anoikis—cells that have lost membrane integrity will show red PI staining throughout the nuclei and a ring-like green staining Annexin V-FITC on the plasma membrane; late stage of Anoikis—cells stained with PI for the nuclei without Annexin V staining with or without halo-green like Annexin V-FITC staining. Apoptosis/Anoikis activation was examined by Caspase 8, 3/7 activity assay: (B) caspase 8 activity significantly increased in cells treated with KIAA0100 siRNA in both 24 and 48 h after transfection compared to mock control cells and cells treated with negative siRNA (*, p < 0.05); (C) caspase 3/7 activities in cells treated with KIAA0100 siRNA were significantly higher compared to mock control cells and cells treated with negative siRNA (*, p < 0.05).