Table 1.
Adenovirus receptor, the residues shown to facilitate receptor interactions, and demonstrated tropism ablating mutations. Prototype viral receptor refers to the adenoviral serotype and protein used in the receptor interaction study for which the binding residues and mutations are described. Named mutations are in bold with the mutations in brackets. amino acids are described with single letter code. Δ indicates a deletion mutation, while substitution mutations are described with the original residue letter codes preceding the residue numbers and then the respective substituted amino acid code.
| Receptor | Prototype Viral Receptor | Receptor Binding Residues | Previously Demonstrated Tropism Aablating Mutations | References |
|---|---|---|---|---|
| CAR | Ad5—Fiber Protein knob | A406; S408; P409; R412; Y477; R481; L485; Y491 | KO1 (SP408-409EA); KO2 (ΔVK441-44s); KO3 (R460E); KO4 (ΔGK509-510); KO5 (ΔGT538-539); KO8 (N468T); KO9 (V466H); KO10 (P505A); KO11 (Δ404-581 Whole region) | Jakubczak et al., 2001 [106] |
| ΔTAYT (ΔTAYT489-492) | Roelvink et al., 1999 [107] | |||
| CD46 | Ad35—Fiber Protein knob | F132; N133; T136; R169; M170; S172; N194; E192 | F242; R279; S282 | Wang et al., 2007 [108] |
| DSG2 | Ad3—Fiber Protein Knob | N186; V189; S190; D261; F265; L292; L296; E299 | N186D; V189G; S190P; D261N; F265L; L296R; E299V; ND186-261DN; ΔD261+L296R; NDL186-261-296DNR. | Wang et al., 2013 [109] |
| GD1a/Sialic acid | Ad37—Fiber Protein knob | Y308; Y312; P317; V322; K322 | None reported | Nilsson et al., 2011 [110] |
| Blood Coagulation Factor X | Ad5—Hexon Protein HVR’s | HVR regions 3; and 7 (Individual residues not clearly defined). | Ad5HVR48 (Ad5 with the HVR’s of Ad48) | Waddington et al., 2008 [111] |
| HVR5-BAP (71aa BAP (Biofilm Associated Protein) peptide insert) | Kalyuzhniy et al., 2008 [112] | |||
| HVR5* (TE268-269AT); HVR7* (ITEL420-422-423-425GNSY); E451Q | Alba et al., 2009 [113] | |||
| HSPG | Ad5—Fiber Protein shaft | KKTK91-94 | S* (KKTK91-94GAGA); KKTK91-94RGDK | Paolo et al., 2007, Kritz et al., 2007 [114,115] |
| Integrin | Ad5—Penton Protein | R340, G341, D342 | RGE (D342E) | Bai et al., 1993 [116] |
| EGD (R340E) | Henning et al., 2005 [117] | |||
| MARCO | Ad5—Hexon Protein | HVR1; implied but not conclusively determined | None reported | Stichling et al., [118] |