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. 2018 Jun 10;16(6):204. doi: 10.3390/md16060204

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of heteronemin on the induction of apoptosis and autophagy in LNcap cells. (A) LNcap cells were treated with heteronemin (0, 1.28, 2.56 and 5.12 μM) for 24 h. The protein expression of activated caspase 3 and cleaved PARP, as well as LC3B II, was analyzed by Western blotting. The GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) was the loading control. The viability (B) and apoptosis (C) of LNcap cells were determined with MTT and flowcytometric analysis after treatment of heteronemin (blue bars), heteronemin + 3-MA (red bars) and heteronemin + CQ (grey bars). Data are expressed as mean ± SD. * p < 0.05; ** p < 0.01; and *** p < 0.001 vs heteronemin group.

Effect of heteronemin on the induction of apoptosis and autophagy in LNcap cells. (A) LNcap cells were treated with heteronemin (0, 1.28, 2.56 and 5.12 μM) for 24 h. The protein expression of activated caspase 3 and cleaved PARP, as well as LC3B II, was analyzed by Western blotting. The GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) was the loading control. The viability (B) and apoptosis (C) of LNcap cells were determined with MTT and flowcytometric analysis after treatment of heteronemin (blue bars), heteronemin + 3-MA (red bars) and heteronemin + CQ (grey bars). Data are expressed as mean ± SD. * p < 0.05; ** p < 0.01; and *** p < 0.001 vs heteronemin group.