Figure 5.

Heteronemin induces apoptosis and MMP disruption in LNcap cells. Cells were treated with the indicated concentrations of heteronemin for 24 h. (A) Apoptosis induction of LNcap and PC3 cells as well as (B) mitochondrial membrane potential of LNcap were assessed with annexin V/PI and rhodamine 123 staining using flow cytometric analysis; (C) the change of nuclear morphology was determined with DAPI staining and observed in LNcap cells using a fluorescent microscope. The results are presented as mean ± SD of three independent experiments (** p < 0.01; *** p < 0.001).