Fig. 4.

35S::AtDICE1 resulted in defects in cellulose biosynthesis. (A) Treatment with isoxaben in WT plants. Plants were grown for 7 d on MS-agar plates containing isoxaben as indicated. Scale bars represent 0.5 mm. (B) Cell wall monosaccharide composition analysis. Inflorescence stems of 60-d-old soil-grown plants were used. AIR, alcohol-insoluble residue. Neutral monosaccharides in the sulfuric acid-soluble fraction were determined by quantification of their corresponding alditol acetates by gas chromatography. Rha, rhamnose; Fuc, fucose; Ara, arabinose; Xyl, xylose; Man, mannose; Gal, galactose; Glu, glucose. Error bars indicate s.e. using triplicate experiments (*P < 0.05; **P < 0.01, unpaired t-test). (C) Observation of cortical microtubule arrangement by confocal laser microscopy. Cortical microtubules were visualized in normal cells from GFP-TuA6 plants (upper) and swollen cells from 35S::AtDICE1/GFP-TuA6 plants (lower). Scale bars indicate 25 αµ.