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. 2018 Jun 11;10(6):192. doi: 10.3390/cancers10060192

Figure 1.

Figure 1

Recombinant type 1 repeat domain (rTSR1) induces caspase-dependent apoptosis in human umbilical vein endothelial cells (HUVECs). (A) SDS-PAGE and immunoblot analysis of purified rTSR1 showed a band at ~10 kDa; (B) rTSR1 dose-dependently induced apoptosis in HUVECs in the presence of VEGF. Data shown are apoptosis observed at 24 h post-1000 nM rTSR1 treatment; (C) Pan-caspase inhibitor (Z-VAD-FMK) significantly reduced the 1000 nM rTSR1-induced apoptosis at 24 h post treatment. Data represent the mean of triplicates ± SD. Statistical analysis performed by one-way ANOVA. * p < 0.05, ** p < 0.01, **** p < 0.0001.