Fig. 11.

Distribution of callose in the subapical region of pollen tubes grown in medium with sucrose or glycerol. In all cases, fluorescence was measured starting from the apex down to 30 μm. (A) In medium with sucrose, distribution of callose did not differ during the transition from 4 to 6 h of germination. (B) Distribution of callose differed when pollen tubes were grown in medium with glycerol, especially after 5 h of growth, because the callose signal stabilized only around 20–25 μm from the apex. (C, D, E) Visualization of callose in pollen tubes grown in medium with glycerol, respectively after 4, 5 and 6 h of germination. Scale bars = 10 μm.