Fig. 12.

Effects of germination medium on the translocation of the generative cell and on the deposition of callose plugs. (A) Ratio between the movement of the generative cell from the grain and the corresponding pollen tube length as measured from 3 to 7 h after germination in growth medium containing either sucrose (S, black bars) or glycerol (G, grey bars). Standard deviation is indicated at the top of the bars. *P < 0.05. (B) Ratio between positions of the first and second callose plugs and the corresponding pollen tube length as determined after 7 h of germination. The first callose plug is the one closest to the pollen grain. Bars indicate standard deviation. (C) A pollen tube grown in BKS for 7 h, in which two callose plugs (arrows) can be observed. Scale bar = 50 μm. (D) A pollen tube grown for 7 h in BKG containing only the first callose plug (arrow). Scale bar = 50 μm.