Fig. 3.

Analysis of metabolites in pollen tubes grown in BK medium supplemented with sucrose or glycerol. The analysis was performed after each hour of germination for a period of 6 h. (A) Measurement of the concentrations of ATP and ADP in pollen tubes grown in BK plus sucrose (BKS) or plus glycerol (BKG). (B) Concentration analysis of UDP-glucose in the two experimental conditions. (C) Analysis of the concentration of glucose and fructose in pollen tubes grown in BK medium plus glycerol. No signal was found at the first hour because values were below the instrumental detection limit. (D) Analysis of glucose and fructose in pollen tubes grown in BK medium plus sucrose. In all cases, bars indicate the standard deviation.