Fig. 2.
Maximum likelihood (ML) phylogeny based on a 486-bp region of the FLAV U1 gene. The phylogenetic tree was rooted with Hart Park Virus (HPVirus) strain AR7C (accession no. KM205011.1) and includes four FLAV sequences from Chicago generated in this study represented with solid underlines (accession nos. MG844997–MG85000) as well as 61 publically available sequences of the Flanders virus U1 gene from the United States (accession nos. KF028661–KF028763). The positive control is highlighted with a dashed underline (accession no. KF028716). Bootstrap values are based on 10,000 ML replicates generated in RAxML (Stamatakis 2014) and values above 50.0 are shown.