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Immunoreactivity of whole-cell lysates from H. pullorum pgl and wbp gene mutants. Whole cell lysates from H. pullorum NCTC 12824 and corresponding pglBCAJH and wbpOS insertional knockout mutants were separated by SDS-PAGE, Western blotted and probed with a polyclonal antibody raised against purified HgpA glycoprotein. Shaded arrowheads indicate position of bands corresponding to putative glycoproteins whilst the unshaded arrowhead indicates the band corresponding to HgpA itself.
