Figure 5.
TFs regulated FXR-FOXO3a-mediated inflammation. (A) Effects of TFs on the expression levels of FOXO3a and NF-κB in the liver on the basis of the immunofluorescence assay. (B) Effects of TFs on the expression levels of FOXO3a and NF-κB in the liver on the basis of the western blotting assay. (C) Effects of TFs on the expression levels of COX-2 (cyclooxygenase-2) and HMGB1 (high- mobility group box 1) on the basis of the western blotting assay. (D) Effects of TFs on the mRNA levels of IL-1β, IL-6, and TNF-αon the basis of the real-time PCR assay. The values are expressed as the mean ± SD (n = 3); ## p < 0.01 versus control group; * p < 0.05 and ** p < 0.01 versus model group.