Table 1.
Sequences of primers, PCR conditions, annealing temperature for amplifying target genes.
| Description | Target Genes | Sequence (5′-3′) | Amplicon Size | Annealing Temp (°C) | Reference |
|---|---|---|---|---|---|
| β-Lactamase | SHV | FP: AGCCGCTTGAGCAAATTAAAC RP: ATCCCGCAGATAAATCACCAC |
713 | 64 | [49] |
| TEM | FP: CATTTCCGTGTCGCCCTTATTC RP: CGTTCATCCATAGTTGCCTGAC |
800 | 62 | ||
| CTX-M-15 | FP: TTAGGAARTGTGCCGCTGYA RP: CGATATCGTTGGTGGTRCCAT |
688 | |||
| CTX-M-27 | FP: TCAAGCCTGCCGATCTGGT RP: TGATTCTCGCCGCTGAAG |
561 | |||
| Plasmid-mediated quinolone resistance | qnrA | FP: AAGGAAGCCGTATGGATATT RP: AGCTAATCCGGCAGCACTAT |
670 | 54 | [50] |
| qnrB | FP: CGACCTGAGCGGCACTGAAT RP: TGAGCAACGATGCCTGGTAG |
515 | |||
| qnrS | FP: ACCTTCACCGCTTGCACATT RP: CCAGTGCTTCGAGAATCAGT |
571 | |||
| oqxA | FP: CTCGGCGCGATGATGCT RP: CCACTCTTCACGGGAGACGA |
280 | 64 | [51] | |
| Horizontal gene transfer | intI1 | FP: CCTCCCGCACGATGATC RP: TCCACGCATCGTCAGGC3 |
280 | 55 | [52] |
| intI2 | FP: CACGGATATGCGACAAAAAGGT RP: GTAGCAAACGAGTGACGAAATG |
788 | 55 | [53] |