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Production of the antimicrobials during growth. SF214 was grown in minimal (S7) medium at 25 °C and the growth curve reported (white symbols). Aliquots of the cell culture were collected at various times, size-fractionated, and tested against the target bacteria. Dashed lines indicate the antimicrobial activity (diameter in mm of inhibition halo) of the fraction larger than 10 kDa against L. monocytogenes (A) and of the fraction smaller than 3 kDa against S. aureus (B).
