Table 3.

Effects of heat, chemicals, and enzymes on antimicrobial activity.

Treatment	Supernatant Activity b < 3 kDa against S. aureus
None	10
Trypsin a	10
Proteinase K a	10
DNase a	10
Ribonuclease A a	10
Acetone c	10
Ethyl alcohol c	10
Chloroform c	10
Toluene c	10
Incubation (15 min) at:
60 °C	10
80 °C	10
100 °C	5

^a The enzyme concentration was 100 μg/mL [20]. ^b Diameter (mm) of the inhibition halo in plate assays. ^c A 10% (v/v) concentration was used.