Figure 1.
Effects of extracts of Betula platyphylla on the differentiation of BMMs into osteoclasts. (A) BMMs were cultured for 4 days with RANKL (10 ng/mL) and M-CSF (30 ng/mL) in the presence of the indicated concentrations of extracts. Total: 80% methanol extract of B. platyphylla barks, n-Butanol: n-butanol fractions of the total, CHCl3: CHCl3 fractions of the total, and H2O: H2O fractions of the total; (B) TRAP activity was measured. *, p < 0.05; **, p < 0.01; ***, p < 0.001 (n = 3).