Figure 5.

Betulin downregulated RANKL-mediated activation of NFATc1 through inhibition of p38 phosphorylation in BMMs. The effects of betulin on RANKL-induced phosphorylation of MAP kinases and degradation of I-kB and NFATc1 were evaluated by Western blot analysis. Actin was used as an internal control. (A) BMMs were pretreated with vehicle (0.1% DMSO) or betulin (3 μM) for 1 h prior to RANKL (10 ng/mL) stimulation at the indicated time periods; (B) BMMs were treated with vehicle (0.1% DMSO) or betulin (3 μM) for 1 h, and then RANKL (10 ng/mL) and M-CSF (30 ng/mL) were treated at the indicated time periods.