Figure 1.
Geldanamycin-induced growth arrest. (A) Cells of three strains, wild-type (Ld wt), vector control (Ld [pcosTL]), and Hsp90-overexpressing cells (Ld [pcos90]) were seeded at 5 × 106 cells/ml and incubated for 24 h at the indicated concentrations of geldanamycin (GA). Cell density was measured, and the mean values from six independent experiments were calculated and plotted against drug concentration. The error bars represent the SD. (B) Leishmania promastigotes from logarithmically growing (25°C - lg) or from stationary (25°C - st) culture as well as cells heat shocked at 37°C or treated with GA (200 μg/ml) were analyzed by flow cytometry. Culture aliquots were removed after 24 h and stained with SYTOX. G1, S, and G2/M refer to the corresponding cell cycle stages. Percentages of cells in respective cell cycle stages are given.