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. 2018 Jun 1;37(3):126–133. doi: 10.1089/mab.2018.0008

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© Robert Hnasko et al. 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons Attribution Noncommercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and the source are cited.

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FIG. 3. — RMH359 hybridoma cell viability is MCM dose dependent. Macrophages were seeded in 15 mL of medium at 1 × 10⁶ cells/80-cm² flasks and grown for either 24 hours () or 72 hours (○) to produce MCM. MCM was diluted into hybridoma growth medium from 0% to 50% and the MTT assay (570 nm AU) was used to determine cell viability after 4 days. The MCM dose–response of viable RMH359 cells was plotted (mean AU ± SEM) and curve fitting performed using a 3^rd-order linear regression (r² > 0.99).

Inline graphic — RMH359 hybridoma cell viability is MCM dose dependent. Macrophages were seeded in 15 mL of medium at 1 × 10⁶ cells/80-cm² flasks and grown for either 24 hours () or 72 hours (○) to produce MCM. MCM was diluted into hybridoma growth medium from 0% to 50% and the MTT assay (570 nm AU) was used to determine cell viability after 4 days. The MCM dose–response of viable RMH359 cells was plotted (mean AU ± SEM) and curve fitting performed using a 3^rd-order linear regression (r² > 0.99).