Fig. 5.

Supervillin activates ERK/p38 signaling in normoxia and hypoxia. a, b. Huh-7 (a) and MHCC-97H (b) cells were transfected with control or supervillin-specific siRNA and exposed to hypoxia for 16 h, and then the changes in phosphorylated p38, ERK, and JNK were detected by western blot. β-tubulin was used as the loading control. c. Quantification of the p-ERK1/2 level in hypoxic Huh-7 and MHCC-97H cells transfected with control or supervillin-specific siRNA. Each p-ERK1/2 level was normalized to the level in hypoxic cells transfected with control siRNA. d. Quantification of the p-p38 level in hypoxic Huh-7 and MHCC-97H cells transfected with control or supervillin-specific siRNA. Each p-p38 level was normalized to the level in hypoxic cells transfected with control siRNA. Data represent the mean of at least three independent experiments ± SD