A253 cells (3.0 × 105) were cultured in the presence or absence of LPS (10 µg/mL) for 24 h, and the supernatant harvested. (A) IL-6, (B) IL-12, (C) MCP-1, (D) RANTES, and (E) GM-CSF were assessed by multiplex array. All samples were analyzed in triplicate and results of three independent experiments are shown (* p < 0.05, ** p < 0.001, *** p < 0.0001, N.S. = non significant).