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. Author manuscript; available in PMC: 2018 Nov 22.
Published in final edited form as: Sci Signal. 2018 May 22;11(531):eaao2341. doi: 10.1126/scisignal.aao2341

Fig. 1. Generation of HEK 293 and U2OS cells for tetracycline-inducible expression of FAM83 proteins.

Fig. 1

A. Schematic representation of the human FAM83 family of proteins and the conserved domain of unknown function, DUF1669, that characterises them. B. A single copy of each FAM83 gene (A–H) tagged with GFP at the N-terminus was stably inserted downstream of a tetracycline-inducible promoter in HEK 293 cells. Cells were treated with doxycycline and lysed at the indicated times after treatment. Extracts were resolved by SDS-PAGE and subjected to immunoblotting (IB) for GFP. ERK1 and ERK2 (ERK1/2) and GAPDH are loading controls. This blot is representative of 2 independent experiments. C. A single copy of each FAM83 gene (A–H) tagged with GFP at the C-terminus was stably inserted downstream of a tetracycline-inducible promoter in U2OS cells. Extracts of doxycycline-induced cells were immunoblotted for GFP and the loading control GAPDH. FAM83B is not included on the blot because we were unable to detect FAM83B-GFP expression in U2OS cells. This blot is representative of 2 independent experiments.