Skip to main content
. Author manuscript; available in PMC: 2018 Nov 22.
Published in final edited form as: Sci Signal. 2018 May 22;11(531):eaao2341. doi: 10.1126/scisignal.aao2341

Fig. 5. FAM83 proteins colocalize with endogenous CK1α in cells.

Fig. 5

U2OS cells stably integrated with Tet-inducible expression of GFP, GFP-FAM83B, GFP-FAM83F, or GFP-FAM83H were treated with doxycycline for 16 h prior to processing cells for fluorescence microscopy to detect GFP and endogenous CK1α (anti- CK1α). DNA was stained with DAPI. Images from one field of view representative of three independent experiments are shown. The number of cells displaying staining patterns identical to the representative image were documented for each experiment: GFP-FAM83B (n=56); GFP-FAM83F (n=60); GFP-FAM83H (n=48); GFP only (n=38); no transgene (n=82). Scale bars, 20 µm.