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. Author manuscript; available in PMC: 2018 Nov 22.
Published in final edited form as: Sci Signal. 2018 May 22;11(531):eaao2341. doi: 10.1126/scisignal.aao2341

Fig. 6. The association between FAM83 proteins and specific CK1 isoforms is selective in cells.

Fig. 6

A. U2OS cells stably integrated with Tet-inducible expression of GFP-FAM83F or GFP-FAM83H were transfected with either mCherry-CK1α (α) or mCherry-CK1ε (ε). GFP-FAM83F and GFP-FAM83H expression was induced with doxycycline for 24 h prior to processing cells for fluorescence microscopy. DNA was stained with DAPI. Images from one field of view representative of three independent experiments are included. The number of cells that displayed staining patterns identical to the representative image were documented for each experiment: GFP-FAM83F + mCherry-CK1α (n=44); GFP-FAM83F + mCherry-CK1ε (n=40); GFP-FAM83H + mcherry-CK1α (n=32); GFP-FAM83H + mCherry-CK1ε (n=40). Scale bar, 20 µm. B. U2OS cells stably integrated with Tet-inducible expression of GFP-FAM83F or GFP-FAM83H were induced with doxycycline for 16 h prior to processing cells for fluorescence microscopy with CK1α (α) or CK1ε (ε) antibodies. Untransfected cells stained with CK1α or CK1ε antibodies were used as negative controls. Images from one field of view representative of three independent experiments are shown. The number of cells that displayed staining patterns identical to the representative image were documented for each experiment: GFP-FAM83F with CK1α (n=60); GFP-FAM83F with CK1ε (n=43); GFP-FAM83H with CK1α (n=48); GFP-FAM83H with CK1ε (n=35); no transgene with CK1α (n=82); no transgene with CK1ε (n=27). Scale bars, 20 µm.