Skip to main content
. Author manuscript; available in PMC: 2018 Nov 22.
Published in final edited form as: Sci Signal. 2018 May 22;11(531):eaao2341. doi: 10.1126/scisignal.aao2341

Fig. 8. FAM83H colocalizes with and contributes to the subcellular localization of endogenous CK1α.

Fig. 8

A. FAM83H-/- U2OS cells were transfected with plasmids encoding either GFP-FAM83H, GFP-FAM83H (D236A), or GFP-FAM83H (F270A). Untransfected knockout (FAM83H-/-) cells were used as controls. Cells were processed for fluorescence microscopy with antibody recognizing CK1α. DNA was stained with DAPI. Images from one field of view representative of 3 independent experiments are included. Scale bar, 10 µm. B. The boxplot shows the range, mean, and lower and upper quartiles of the Pearson’s correlation coefficients of GFP-FAM83H and endogenous CK1α intensities within above-background pixels in the cytoplasm. C. GFP-FAM83H constructs were transfected into FAM83H-/- U2OS cells, and extracts were immunoblotted (IB) with the indicated antibodies. Untransfected wild-type (WT) cells were used as controls. This blot is representative of three independent experiments.