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. 2018 Jun 29;13(6):e0199935. doi: 10.1371/journal.pone.0199935

Fig 2. Sodium dodecyl sulfate polyacrylamide gel of lysate of Escherichia coli cells expressing Ab-CB-1 from the plasmid vector pET-28a (+).

Fig 2

M: PageRuler Prestained Protein Ladder (Thermo, USA); 1, 2: Protein extracts from induced E. coli cells harboring the empty pET-28a (+) vector by 0.5 and 1.0 mM IPTG; 3: Protein extract from non-induced E. coli cells containing the Ab-CB-1 construct; 4,5: Protein extracts from E. coli cells containing the Ab-CB-1 construct and induced by 0.5 and 1.0 mM IPTG; 6: Supernatant of cell lysate from E. coli cells containing the Ab-CB-1 construct; 7 Purified recombinant Ab-CB-1.